In Vivo Microdialysis and HPLC 1) Microdialysis Collection Protocol Probes& Collection Setup: CMA10 (CarnegieMedicin) dialysis probes pictured at left are used in our laboratory to collect dialysates from discrete brain nuclei. Few microdialysis studies have actually directly compared the in vitro and in vivo characteristics of the same microdialysis probes, and the results of these were inconsistent.
Sam and Justice (1996) reported that E d values were less in vitro than in vivo in the striatum of the anesthetized rat, but the same probes were not used in both experiments.
Learn microdialysis in the comfort of your own lab. Laboratory Training Manual of Microdialysis and In Vivo UF Sampling. Chapter 1: Overview of In Vivo Bioanalytical Sampling Techniques. 11. The Ideal Tool; 12. Open Cannula Techniques; 13. Microdialysis; 14. Ultrafiltration; Chapter 2: Preparation for Microdialysis.
21. Selecting a Probe; 22. Microdialysis has been used for over 30 years to sample molecules within the extracellular space of a tissue, including brain and muscle. One advantage of the technique is that it can be performed in awake, behaving animals so that longitudinal measurements can be performed under physiological conditions. Microdialysis is an important in vivo sampling technique, useful in the assay of extracellular tissue fluid. The technique has both preclinical and clinical applications but The technique of in vivo microdialysis allows for continuous sampling of components of the extracellular fluid in specific brain areas of awake, freely moving animals.
In vivo microdialysis Microdialysis is a widely used technique for in vivo testing of endogenous molecules, drug effects, and other vital processes. The technique is minimally invasive, allowing the continuous and free flow of solutes through a probe that is designed to function much like a Microdialysis is the only sampling technique that can continuously monitor drug, biochemical, or metabolite concentrations in vivo in virtually any tissue.
The technique can be limited by the probes recovery, which is directly dependent on the volume of the probe and the flow rate. Microdialysis Systems. Automated Rodent Microdialysis Systems are manufactured utilizing either the Swivel or Raturn principle and offer a variety of configurations from which to choose, depending on your research needs.
There is flexibility to integrate optional